Toxi-ChromoPlate

Based on a novel mutant of E.coli, sensitive to a wide spectrum of toxic substances.

 

The procedure exposes the bacteria to the toxicants in the sample for a short (90-minute) incubation period. After the incubation period, a chromogenic substrate is added. If the sample is toxic, no colour will develop and if the sample is non-toxic, a distinctive blue colour quickly develops. The kit provides a clear, completely objective measurement of the toxicity of the sample by a simple visual qualitative evaluation of the colour obtained, or quantitatively by spectrophotometry using a micro-plate reader.

Toxi-ChromoTest kit contains three sets of components and, therefore, allows four separate testing procedures to be completed at the same or different times.

 

In 2006, the Toxi-ChromoTest was verified by the ETV program under the U.S. Environmental Protection Agency.

Characteristics Each Toxi-ChromoTest kit can be used for a total of 192 tests. These tests must include standards and controls (using at least 32 units.) Thus, a single kit can screen 160 samples for yes/no response without replicates. It is necessary to allow sample dilutions and replicates, thus testing of no more than 20 samples with each kit for reliable and quantitative bioassays is recommended.	Applications Applications for the Toxi-ChromoTest include screening of effluents for changes in toxicity levels, monitoring of municipal discharges for occasional spills, inclusion in a "battery of tests" for monitoring aqueous environmental samples, detection of potential toxicity in raw materials, and use as a convenient research and teaching tool. Used within Universities across North America, the Toxi-ChromoTest kit can be tailored to meet the demands of any university student. Used in support of a graduate thesis, or as a learning tool for laboratory use at the undergraduate level.
Two Fold Dilutions Column 1 – reagent blank no colour  Columns 2&3 – HgCL2 positive control Columns 4,5,6 – test material A-3 replicates toxic at 30 ppb non toxic at 4ppb. Columns 7,8,9-test material B-3 replicates toxic at 1 ppm, non toxic at 0.25 ppm Columns 10,11,12 – material C- 3 replicates non toxic at concentrations tested (2ppm)

(EPA) Environmental Technology Veriication (ETV) program.