The SOS-ChromoTest kit is based on a novel genetically engineered E.coli, which measures the primary response of a cell to genetic damage. In just a few hours, the kit provides a clear, completely objective measurement of the genotoxicity of a sample by a simple visual qualitative evaluation of the degree of DNA damage the cell experiences by observing the colour obtained. A more quantitative value may be obtained by spectrophotometry using a micro-plate reader. The SOS-Chromotest analyses are highly correlated with the Salmonella Ames test for certain materials.
SOS- ChromoPad
The SOS-ChromoPad Test, a solid phase test version of the SOS ChromoTest, is a bioassay screening tool used to determine acute genotoxicity in soils, sediments, sludge or other high water content waste material directly, without extraction. The kit can also be used to analyze aqueous samples high in suspended solids.
Characteristics
For quantitative measurements, it is often necessary to allow for an adequate range of sample dilutions and replicates, thus testing five to ten samples with each kit. Below is an example of the SOS-ChromoTest kit testing results for different genotoxic materials.
Column 1 is a blank showing the normal background color produced by the SOS strain in the absence of any genotoxic materials Columns 2-7 are replicates of exposure to a standard genotoxic positive control showing a strong genotoxic response in rows 1-3, a weak genotoxic response in row 4-6 and no genotoxic response in row7 and 8.
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ApplicationsPotential applications of the SOS-ChromoTest that take advantage of the genetically engineered strain of E. coli, including testing of effluents for possible mutagenic or carcinogenic genotoxic compounds. It provides for an evaluation of potential genotoxicity, and is a convenient research tool. SOS-ChromoTest offers the following advantages over traditional methods
The SOS-Chromotest kit has been in use since 1982, has been evaluated by hundreds of compounds and cited in over 150 publications. The SOS Chromotest is available with or without the S9 Activation Enzymes
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A single SOS-ChromoTest kit can be used for a total of 96 unit tests. These tests must include standards and controls (using at least 16 units). Thus, a single kit can screen 80 samples for yes/no responses.
